The University of Texas Medical Branch
Department of Biochemistry and Molecular Biology Sealy Center for Structural Biology Computational Biology



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                SDAP - Structural Database of Allergenic Proteins
Go to: SDAP All allergens       Go to: SDAP Food allergens
Send a comment to Werner Braun      Submit new allergen information to SDAP
  
Alphabetical listing of allergens: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Access to SDAP is available free of charge for Academic and non-profit use.
Licenses for commercial use can be obtained by contacting W. Braun (webraun@utmb.edu).
Secure access to SDAP is available from https://fermi.utmb.edu/SDAP
Allergen Info Description
Allergen ID 331
Allergen Scientific Name Act d 1
Allergen Species - Systematic Name Actinidia deliciosa
Allergen Species - Common Name Kiwi fruit
Allergen Common source foods
Allergen Common name
Allergen Keywords Cysteine protease; EC 3.4.22.14; Old Name: Act c 1
Allergen IUIS (Yes=1, No=0) 1
ID Allergen Name
Act d 1 - References
ID Reference
Pubmed ID: 9564807
Pastorello EA, Conti A, Pravettoni V, Farioli L, Rivolta F, Ansaloni R, Ispano M, Incorvaia C, Giuffrida MG, Ortolani C, Identification of actinidin as the major allergen of kiwi fruit. J Allergy Clin Immunol. 1998 Apr;101(4 Pt 1):531-7.
Act d 1 - Protein Sequences
Source Link to source
SwissProt P00785
GenBank 166317
Act d 1 - PDB Structure
PDB Id PDB Name PDB Entry Deposition Date Experiment Type Observation
331 1 2ACT 27-NOV-79 X-ray diffraction resolution 1.7 A
331 231 1AEC 1992-02-05 X-ray diffraction resolution 1.86 A
1AEC.pdb


Act d 1 - Theoretical Model Structure
Model ID PDB file PDB Snapshot PDB Snapshot 90 PDB Snapshot 180 PDB Snapshot 270 3D PDB View
2 GetModel Model Pic Model Pic Model Pic Model Pic Click here
615 GetModel Model Pic Model Pic Model Pic Model Pic Click here
Act d 1 - Sequence Search
Sequence Header Sequence
Act d 1
SDAP Sequence ID: 2
SDAP Allergen ID: 331
MGLPKSFVSMSLLFFSTLLILSLAFNAKNLTQRTNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNADTNRSYKVGLNQFADLTDEEFRSTYLGFTSGSNKTKVSNRYEPRFGQVLPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVTGVLISLSEQELIDCGRTQNTRGCNGGYITDGFQFIINNGGINTEENYPYTAQDGECNLDLQNEKYVTIDTYENVPYNNEWALQTAVTYQPVSVALDAAGDAFKHYSSGIFTGPCGTAIDHAVTIVGYGTEGGIDYWIVKNSWDTTWGEEGYMRILRNVGGAGTCGIATMPSYPVKYNNQNHPKPYSSLINPPAFSMSKDGPVGVDDGQRYSA
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
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Act d 1
SDAP Sequence ID: 615
SDAP Allergen ID: 331
MGLPKSFVSMSLLFFSTLLILSLAFNAKNLTQRTNDEVKAMYESWLIKYGKSYNSLGEWERRFEIFKETLRFIDEHNADTNRSYKVGLNQFADLTDEEFRSTYLGFTSGSNKTKVSNRYEPRVGQVLPSYVDWRSAGAVVDIKSQGECGGCWAFSAIATVEGINKIVTGVLISLSEQELIDCGRTQNTRGCNGGYITDGFQFIINNGGINTEENYPYTAQDGECNVELQNEKYVTIDTYENVPYNNEWALQTAVTYQPVSVALDAAGDAFKQYSSGIFTGPCGTAIDHAVTIVGYGTEGGIDYWIVKNSWDTTWGEEGYMRILRNVGGAGTCGIATMPSYPVKYNNQNYPEPYSSLINPPAFSMSKDGPVGVEDGQRYSA
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Expasy Blast Search
Act d 1 - Statistics from Alphafold ( SI is sequence id, Best template format: PDB_ChainID)
Allergen Name pLDDT Score pTM Score Best PDB template %Seq ID Align length Seq length Query Range Sequence Range E-Value Bit Score Download Model
Act d 1 87.9 0.79 1AEC_A 99.541 218 380 127 - 344 1 - 218 0 449 Download (SI: 615)
Act d 1 87.65 0.78 1AEC_A 99.083 218 380 127 - 344 1 - 218 0 449 Download (SI: 2)
Act d 1 - Alphafold Theoretical Model Structure
Model Structure
615_relaxed_rank_1_model_4.pdb


Coverage Plddt
Act d 1 - Protein Sequence Properties!
Protein Sequence Protease cleavage sites PeptideCutter at ExPASy
P00785 Click here to process the data
166317 Click here to process the data
Act d 1 - PFAM Information
SeqID Aller ID AlnS AlnE EnvS EnvE PFAM ID Family Type Clan Description
2 331 42 99 42 99 PF08246 Inhibitor_I29 Domain No_clan Cathepsin propeptide inhibitor domain (I29)
2 331 127 342 127 342 PF00112 Peptidase_C1 Domain CL0125 Papain family cysteine protease
615 331 42 99 42 99 PF08246 Inhibitor_I29 Domain No_clan Cathepsin propeptide inhibitor domain (I29)
615 331 127 342 127 342 PF00112 Peptidase_C1 Domain CL0125 Papain family cysteine protease
Where: AlnS= Alignment start position, AlnE= Alignment end position, EnvS= Envelope start position, EnvE= Envelope end position.

Welcome

Predicted B-Cell epitope using BCEP method for Act d 1

Probe radius : 1.400

POLAR area/energy = 7638.76

APOLAR area/energy = 13116.00

Total area/energy = 20754.75

Number of surface atoms = 1653

Number of buried atoms = 1318

Residues Predicted with high probability (in top five patches) are: 1,2,49,51,90,121,246,247,248,251,243,244,245,114,195,197,198 , and medium probability (next top five patches) are : 198,201,239,241,127,185,186,187,216,217,218,219,220,222,119,166,256 . Predicted residues are shown in red and green color stick representation.

Original PDB file 1317.test.pdb
Original PDB file with surface residue only. All amino acid residue are replaced by their CB atom, in case of GLY CA atom is used. Solvent accessible surface area of each residue is stored in last column of the PDB file. 11317.test.txt
Patch drawn around the residues by patch analysis are stored in this file
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area), Col 4: Sum(Psur*Area), Col 5: Total Residue, Col 6: Interface score, Col 7: Surface Score, Col 8: Max Interface Score, Col 9: Minimum Interface Score
1317.test.tmppat
Residues predicted by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Res Number Col 4: Neigbouring Res Name, Col 5: Sum(Pint*Area). Col 6: Sum(PSur*Area), Col 7: Sum(Area), Col 8: Similar no of residue in patch (e.g CYS, CYS = 2)
1317.test.res
All residues scored by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area)/sum(Area), Col 4: Sum(Psur*Area)/sum(Area), Col 5: Total Residue
1317.test.resALL

[For BCEP predicted results, please cite our websites http://fermi.utmb.edu/ http://curie.utmb.edu/ for your future publications (Negi et al., Bioinformatics, 23, 3397-3399, 2007 , Negi et al., J. Mol. Model., 13, 1157-1167, 2007; Negi et al., J. Mol. Model., 12, 921-929, 2006)]



List of B-cell epitopes predicted by BepiPred method using a window size of 9 amino acids
Peptides predicted from BepiPred method can be used to search related peptides in SDAP using PD search and Cross-React method.
Please note: BepiPred Epitopes are predicted using BepiPred webserver implemented in IEDB Analysis Resource at http://tools.iedb.org/main/bcell/
Cleck here for epitopes predicted by BepiPred method

Act d 1 - Epitope Information from published data
No. Position Epitope Sequence Epitope Type View epitope Search epitope in SDAP PD search in SDAP Cross-React search
No epitope information found!
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