The University of Texas Medical Branch
Department of Biochemistry and Molecular Biology Sealy Center for Structural Biology Computational Biology


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                SDAP - Structural Database of Allergenic Proteins
Go to: SDAP All allergens       Go to: SDAP Food allergens
Send a comment to Werner Braun      Submit new allergen information to SDAP
  
Alphabetical listing of allergens: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Access to SDAP is available free of charge for Academic and non-profit use.
Licenses for commercial use can be obtained by contacting W. Braun (webraun@utmb.edu).
Secure access to SDAP is available from https://fermi.utmb.edu/SDAP
Allergen Info Description
Allergen ID 70
Allergen Scientific Name Jun a 1.010101
Allergen Species - Systematic Name Juniperus ashei
Allergen Species - Common Name mountain cedar
Allergen Common source tree
Allergen Common name Pinales
Allergen Keywords Pectate lyase
Allergen IUIS (Yes=1, No=0) 1
Jun a 1.010101 - References
ID Reference
Pubmed ID: 10482836
Midoro-Horiuti T, Goldblum RM, Kurosky A, Wood TG, Schein CH, Brooks EG, Molecular cloning of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1. J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):613-7.
Pubmed ID: 10482835
Midoro-Horiuti T, Goldblum RM, Kurosky A, Goetz DW, Brooks EG, Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1. J Allergy Clin Immunol. 1999 Sep;104(3 Pt 1):608-12.
Pubmed ID: 14563374
Midoro-Horiuti T, Mathura V, Schein CH, Braun W, Yu S, Watanabe M, Lee JC, Brooks EG, Goldblum RM. Major linear IgE epitopes of mountain cedar pollen allergen Jun a 1 map to the pectate lyase catalytic site. Mol Immunol. 2003 Dec;40(8):555-62.
Jun a 1.010101 - Protein Sequences
Source Link to source
SwissProt P81294
Jun a 1.010101 - PDB Structure
PDB Id PDB Name PDB Entry Deposition Date Experiment Type Observation
70 179 1PXZ 07-JUL-03 X-ray diffraction resolution 1.70 A
1PXZ.pdb


Jun a 1.010101 - Sequence Search
Sequence Header Sequence
Jun a 1.010101
SDAP Sequence ID: 165
SDAP Allergen ID: 70
MASPCLIAVLVFLCAIVSCYSDNPIDSCWRGDSNWDQNRMKLADCAVGFGSSTMGGKGGDFYTVTSTDDNPVNPTPGTLRYGATREKALWIIFSQNMNIKLKMPLYVAGHKTIDGRGADVHLGNGGPCLFMRKVSHVILHSLHIHGCNTSVLGDVLVSESIGVEPVHAQDGDAITMRNVTNAWIDHNSLSDCSDGLIDVTLGSTGITISNNHFFNHHKVMLLGHDDTYDDDKSMKVTVAFNQFGPNAGQRMPRARYGLVHVANNNYDPWNIYAIGGSSNPTILSEGNSFTAPSESYKKEVTKRIGCESPSACANWVWRSTRDAFINGAYFVSSGKTEETNIYNSNEAFKVENGNAAPQLTKNAGVVT
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Expasy Blast Search
Jun a 1.010101 - Statistics from Alphafold ( SI is sequence id, Best template format: PDB_ChainID)
Allergen Name pLDDT Score pTM Score Best PDB template %Seq ID Align length Seq length Query Range Sequence Range E-Value Bit Score Download Model
Jun a 1.010101 94.75 0.85 1PXZ_A 100 346 367 22 - 367 1 - 346 0 721 Download (SI: 165)
Jun a 1.010101 - Alphafold Theoretical Model Structure
Model Structure
165_relaxed_rank_1_model_1.pdb


Coverage Plddt
Jun a 1.010101 - Protein Sequence Properties!
Protein Sequence Protease cleavage sites PeptideCutter at ExPASy
P81294 Click here to process the data
Jun a 1.010101 - PFAM Information
SeqID Aller ID AlnS AlnE EnvS EnvE PFAM ID Family Type Clan Description
165 70 104 289 93 289 PF00544 Pectate_lyase_4 Repeat CL0268 Pectate lyase
Where: AlnS= Alignment start position, AlnE= Alignment end position, EnvS= Envelope start position, EnvE= Envelope end position.

Welcome

Predicted B-Cell epitope using BCEP method for Jun a 1.010101

Probe radius : 1.400

POLAR area/energy = 6258.74

APOLAR area/energy = 9171.73

Total area/energy = 15430.47

Number of surface atoms = 1391

Number of buried atoms = 1404

Residues Predicted with high probability (in top five patches) are: 216,217,218,249,270,119,121,143,145,188,75,76,77,81,74,85,52,54 , and medium probability (next top five patches) are : 54,57,58,110,136,181,98,124,194,221,250,100,125,146,40,59,212,214 . Predicted residues are shown in red and green color stick representation.

Original PDB file 1362.test.pdb
Original PDB file with surface residue only. All amino acid residue are replaced by their CB atom, in case of GLY CA atom is used. Solvent accessible surface area of each residue is stored in last column of the PDB file. 11362.test.txt
Patch drawn around the residues by patch analysis are stored in this file
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area), Col 4: Sum(Psur*Area), Col 5: Total Residue, Col 6: Interface score, Col 7: Surface Score, Col 8: Max Interface Score, Col 9: Minimum Interface Score
1362.test.tmppat
Residues predicted by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Res Number Col 4: Neigbouring Res Name, Col 5: Sum(Pint*Area). Col 6: Sum(PSur*Area), Col 7: Sum(Area), Col 8: Similar no of residue in patch (e.g CYS, CYS = 2)
1362.test.res
All residues scored by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area)/sum(Area), Col 4: Sum(Psur*Area)/sum(Area), Col 5: Total Residue
1362.test.resALL

[For BCEP predicted results, please cite our websites http://fermi.utmb.edu/ http://curie.utmb.edu/ for your future publications (Negi et al., Bioinformatics, 23, 3397-3399, 2007 , Negi et al., J. Mol. Model., 13, 1157-1167, 2007; Negi et al., J. Mol. Model., 12, 921-929, 2006)]



List of B-cell epitopes predicted by BepiPred method using a window size of 9 amino acids
Peptides predicted from BepiPred method can be used to search related peptides in SDAP using PD search and Cross-React method.
Please note: BepiPred Epitopes are predicted using BepiPred webserver implemented in IEDB Analysis Resource at http://tools.iedb.org/main/bcell/
Cleck here for epitopes predicted by BepiPred method

Jun a 1.010101 - Epitope Information from published data
No. Position Epitope Sequence Epitope Type View epitope Search epitope in SDAP PD search in SDAP Cross-React search
1 92-104 IFSQNMNIKLKMP IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
2 239-250 AFNQFGPNAGQR IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
3 251-258 MPRARYGL IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
4 317-327 WRSTRDAFING IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
165_relaxed_rank_1_model_1.pdb



Epitope Reference ( ID 26): T. Midoro-Horiuti, V. Mathura, C. H. Schein, W. Braun, S. Yu, M. Watanabe, J. C. Lee, E. G. Brooks, and R. M. Goldblum, Major linear IgE epitopes of mountain cedar pollen allergen Jun a 1 map to the pectate lyase catalytic site, Mol. Immunol. 2003, 40, 555-562.
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