The University of Texas Medical Branch
Department of Biochemistry and Molecular Biology Sealy Center for Structural Biology Computational Biology


SDAP Home Page
SDAP Overview

Search SDAP
SDAP All
SDAP Food

SDAP Tools
FAO/WHO Allergenicity Test
FASTA Search in SDAP
Peptide Match
Peptide Similarity
Peptide-Protein PD Index
Aller_ML, Allergen Markup Language
List SDAP

About SDAP
General Information
Manual
FAQ
Publications
Who Are We
Advisory Board
New Allergen Submission form

Allergy Links

Our Software Tools
MPACK
FANTOM
GETAREA
InterProSurf
EpiSearch

Allergen Databases
WHO/IUIS Allergen Nomenclature database
FARRP Allergen Protein Database (University of Nebraska)
Allergen Database for Food Safety (ADFS)
COMPARE database
ALLFAM (Medical University of Vienna)
Allermatch (Wageninen University)
Allergome Database

Protein Databases
PDB
MMDB - Entrez
SWISS-PROT
NCBI - Entrez
PIR

Protein Classification
CATH
FSSP
iProClass
ProtoMap
SCOP
VAST

Bioinformatics Servers
BLAST @ NCBI
FASTA @ EMBL-EBI
Peptide Match @ PIR
ClustalW @ EMBL - EBI


         SDAP 2.0 - Structural Database of Allergenic Proteins
Go to: SDAP All allergens       Go to: SDAP Food allergens
Send a comment to Werner Braun      Submit new allergen information to SDAP
  
Alphabetical listing of allergens: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Access to SDAP is available free of charge for Academic and non-profit use. Licenses for commercial use can be obtained by contacting W. Braun (webraun@utmb.edu).Secure access to SDAP is available from https://fermi.utmb.edu/SDAP

FAO/WHO Allergenicity Rules based on Sequence Homology

The FAO/WHO allergenicity rules are presented in the Report of a Joint FAO/WHO Expert Consultation on Allergenicity of Foods Derived from Biotechnology, 22-25 January 2001. As proposed in the FAO/WHO Report, cross-reactivity between a query protein and a known allergen has to be considered when there is:
(a) more than 35% identity in the amino acid sequence of the expressed protein, using a window of 80 amino acids and a suitable gap penalty
   or:
(b) identity of 6 contiguous amino acids.

SDAP offers a flexible interface to check the FAO/WHO allergenicity rules against all allergens from the SDAP database. The user should follow the following steps:
1. Enter the name of the sequence
2. Paste or type your protein sequence as a string of single-letter amino acid codes (lower-case amino acid codes will be translated into upper-case amino acid codes). The maximum length of the sequence is 1000; sequences longer than 1000 amino acids will be truncated after the first 1000 amino acids. Besides the codes of the 20 amino acids, acceptable symbols are B (aspartate or asparagine), U (selenocysteine), and X (any amino acid). The symbols Z, *, -, and . are translated into X (FASTA deletes these symbols from its input). Any other input characters (including space and new line) will be deleted.
3. Perform a full FASTA alignment between the user sequence and all allergens from the SDAP database. Although this step is not part of the FAO/WHO allergenicity rules, it should be used to identify allergens that are highly similar with the query sequence. The user can change the maximum E score for the results of the full FASTA alignments.
4. Perform FASTA alignments for an 80 amino acids sliding window along the user sequence. The user can change the sequence identity cutoff from the value proposed by the FAO/WHO allergenicity rules (35%).
5. Perform an exact match search for contiguous amino acids. The FAO/WHO allergenicity rules recommend a number of six contiguous amino acids, but the user can change this value.

Enter sequence name
Paste or type your sequence

Select the allergenicity test:
Full FASTA alignment   
Maximum E score for the results of the full FASTA alignment. Sequences with E values < 0.01 are almost always homologous.
FASTA alignments for an 80 amino acids sliding window   
Sequence identity cutoff used for the 80 amino acids sliding window alignments
Exact match for contiguous amino acids   
Number of contiguous amino acids
FASTA search for contiguous amino acids   
Number of contiguous amino acids


Search made with FASTA 3.45
SDAP Home Page | Search SDAP | SDAP Manual | SDAP FAQ | Contact  
UTMB | Search | Directories | UTMB Map | News | Employment | Sitemap 
This site published by Surendra Negi
Copyright   2001-2023  The University of Texas Medical Branch. Please review our privacy policy and Internet guidelines.