The University of Texas Medical Branch
Department of Biochemistry and Molecular Biology Sealy Center for Structural Biology Computational Biology


SDAP Home Page
SDAP Overview

Use SDAP
SDAP All
SDAP Food

SDAP Tools
FAO/WHO Allergenicity Test
FASTA Search in SDAP
Peptide Match
Peptide Similarity
Peptide-Protein PD Index
Aller_ML, Allergen Markup Language
List SDAP

About SDAP
General Information
Manual
FAQ
Publications
Who Are We
Advisory Board

Allergy Links

Our Software Tools
MPACK
FANTOM
GETAREA
InterProSurf
EpiSearch

Allergen Databases
WHO/IUIS Allergen Nomenclature database
FARRP Allergen Protein Database (University of Nebraska)
Allergen Database for Food Safety (ADFS)
COMPARE database
ALLFAM (Medical University of Vienna)
Allermatch (Wageninen University)

Protein Databases
PDB
MMDB - Entrez
SWISS-PROT
NCBI - Entrez
PIR

Protein Classification
CATH
FSSP
iProClass
ProtoMap
SCOP
VAST

Bioinformatics Servers
BLAST @ NCBI
FASTA @ EMBL-EBI
Peptide Match @ PIR
ClustalW @ EMBL - EBI


                SDAP - Structural Database of Allergenic Proteins
Go to: SDAP All allergens       Go to: SDAP Food allergens
Send a comment to Werner Braun      Submit new allergen information to SDAP
  
Alphabetical listing of allergens: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Access to SDAP is available free of charge for Academic and non-profit use.
Licenses for commercial use can be obtained by contacting W. Braun (webraun@utmb.edu).
Secure access to SDAP is available from https://fermi.utmb.edu/SDAP
Allergen Info Description
Allergen ID 324
Allergen Scientific Name Ara h 1
Allergen Species - Systematic Name Arachis hypogaea
Allergen Species - Common Name peanut
Allergen Common source foods
Allergen Common name
Allergen Keywords Vicilin; Clone P17
Allergen IUIS (Yes=1, No=0) 1
ID Allergen Name
Ara h 1 - References
ID Reference
Pubmed ID: 7560062
Burks AW, Cockrell G, Stanley JS, Helm RM, Bannon GA. Recombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity.J Clin Invest. 1995 Oct;96(4):1715-21.
Pubmed ID: 10998264
Kolarich D, Altmann F. N-Glycan analysis by matrix-assisted laser desorption/ionization mass spectrometry of electrophoretically separated nonmammalian proteins: application to peanut allergen Ara h 1 and olive pollen allergen Ole e 1. Anal Biochem. 2000 Oct 1;285(1):64-75.
Ara h 1 - Protein Sequences
Source Link to source
SwissProt P43237
SwissProt P43238
Ara h 1 - PDB Structure
PDB Id PDB Name PDB Entry Deposition Date Experiment Type Observation
324 272 3S7E 2011-05-26 X-ray diffraction resolution 2.71 A
324 273 3S7I 2011-05-26 X-ray diffraction resolution 2.35 A
324 303 3SMH 2011-06-28 X-ray diffraction resolution 2.43 A crystal structure of the major peanut allergen Ara h 1
3SMH.pdb


Ara h 1 - Theoretical Model Structure
Model ID PDB file PDB Snapshot PDB Snapshot 90 PDB Snapshot 180 PDB Snapshot 270 3D PDB View
34 GetModel Model Pic Model Pic Model Pic Model Pic Click here
35 GetModel Model Pic Model Pic Model Pic Model Pic Click here
Ara h 1 - Sequence Search
Sequence Header Sequence
Ara h 1
SDAP Sequence ID: 34
SDAP Allergen ID: 324
MRGRVSPLMLLLGILVLASVSATQAKSPYRKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDTGATNQRHPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAEPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSEVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSKQFQNLQNHRIVQIEARPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRRSTRSSDNEGVIVKVSKEHVQELTKHAKSVSKKGSEEEDITNPINLRDGEPDLSNNFGRLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREQEWEEEEEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQRESHFVSARPQSQSPSSPEKEDQEEENQGGKGPLLSILKAFN
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Expasy Blast Search
Ara h 1
SDAP Sequence ID: 35
SDAP Allergen ID: 324
MRGRVSPLMLLLGILVLASVSATHAKSSPYQKKTENPCAQRCLQSCQQEPDDLKQKACESRCTKLEYDPRCVYDPRGHTGTTNQRSPPGERTRGRQPGDYDDDRRQPRREEGGRWGPAGPREREREEDWRQPREDWRRPSHQQPRKIRPEGREGEQEWGTPGSHVREETSRNNPFYFPSRRFSTRYGNQNGRIRVLQRFDQRSRQFQNLQNHRIVQIEAKPNTLVLPKHADADNILVIQQGQATVTVANGNNRKSFNLDEGHALRIPSGFISYILNRHDNQNLRVAKISMPVNTPGQFEDFFPASSRDQSSYLQGFSRNTLEAAFNAEFNEIRRVLLEENAGGEQEERGQRRWSTRSSENNEGVIVKVSKEHVEELTKHAKSVSKKGSEEEGDITNPINLREGEPDLSNNFGKLFEVKPDKKNPQLQDLDMMLTCVEIKEGALMLPHFNSKAMVIVVVNKGTGNLELVAVRKEQQQRGRREEEEDEDEEEEGSNREVRRYTARLKEGDVFIMPAAHPVAINASSELHLLGFGINAENNHRIFLAGDKDNVIDQIEKQAKDLAFPGSGEQVEKLIKNQKESHFVSARPQSQSQSPSSPEKESPEKEDQEEENQGGKGPLLSILKAFN
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Expasy Blast Search
Ara h 1 - Statistics from Alphafold ( SI is sequence id, Best template format: PDB_ChainID)
Allergen Name pLDDT Score pTM Score Best PDB template %Seq ID Align length Seq length Query Range Sequence Range E-Value Bit Score Download Model
Ara h 1 75.1 0.64 3S7E_A 96.667 420 614 164 - 581 2 - 418 0 817 Download (SI: 34)
Ara h 1 74.35 0.64 3S7E_A 99.761 418 626 169 - 586 1 - 418 0 853 Download (SI: 35)
Ara h 1 - Alphafold Theoretical Model Structure
Model Structure
35_relaxed_rank_1_model_3.pdb


Coverage Plddt
Ara h 1 - Protein Sequence Properties!
Protein Sequence Protease cleavage sites PeptideCutter at ExPASy
P43237 Click here to process the data
P43238 Click here to process the data
Ara h 1 - PFAM Information
SeqID Aller ID AlnS AlnE EnvS EnvE PFAM ID Family Type Clan Description
34 324 194 324 174 327 PF00190 Cupin_1 Domain CL0029 Cupin
34 324 391 558 390 566 PF00190 Cupin_1 Domain CL0029 Cupin
35 324 206 330 180 333 PF00190 Cupin_1 Domain CL0029 Cupin
35 324 399 563 398 571 PF00190 Cupin_1 Domain CL0029 Cupin
Where: AlnS= Alignment start position, AlnE= Alignment end position, EnvS= Envelope start position, EnvE= Envelope end position.

Welcome

Predicted B-Cell epitope using BCEP method for Ara h 1

Probe radius : 1.400

POLAR area/energy = 21764.91

APOLAR area/energy = 27266.32

Total area/energy = 49031.23

Number of surface atoms = 3379

Number of buried atoms = 1641

Residues Predicted with high probability (in top five patches) are: , and medium probability (next top five patches) are : . Predicted residues are shown in red and green color stick representation.

Original PDB file 2098.test.pdb
Original PDB file with surface residue only. All amino acid residue are replaced by their CB atom, in case of GLY CA atom is used. Solvent accessible surface area of each residue is stored in last column of the PDB file. 12098.test.txt
Patch drawn around the residues by patch analysis are stored in this file
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area), Col 4: Sum(Psur*Area), Col 5: Total Residue, Col 6: Interface score, Col 7: Surface Score, Col 8: Max Interface Score, Col 9: Minimum Interface Score
2098.test.tmppat
Residues predicted by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Res Number Col 4: Neigbouring Res Name, Col 5: Sum(Pint*Area). Col 6: Sum(PSur*Area), Col 7: Sum(Area), Col 8: Similar no of residue in patch (e.g CYS, CYS = 2)
2098.test.res
All residues scored by patch analysis are stored in this file.
How to read: Col 1: Patch No. Col 2: Center Res Name. Col 3: Sum(Pint*Area)/sum(Area), Col 4: Sum(Psur*Area)/sum(Area), Col 5: Total Residue
2098.test.resALL

[For BCEP predicted results, please cite our websites http://fermi.utmb.edu/ http://curie.utmb.edu/ for your future publications (Negi et al., Bioinformatics, 23, 3397-3399, 2007 , Negi et al., J. Mol. Model., 13, 1157-1167, 2007; Negi et al., J. Mol. Model., 12, 921-929, 2006)]



List of B-cell epitopes predicted by BepiPred method using a window size of 9 amino acids
Peptides predicted from BepiPred method can be used to search related peptides in SDAP using PD search and Cross-React method.
Please note: BepiPred Epitopes are predicted using BepiPred webserver implemented in IEDB Analysis Resource at http://tools.iedb.org/main/bcell/
Cleck here for epitopes predicted by BepiPred method

Ara h 1 - Epitope Information from published data
No. Position Epitope Sequence Epitope Type View epitope Search epitope in SDAP PD search in SDAP Cross-React search
1 25-34 AKSSPYQKKT IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
2 48-57 QEPDDLKQKA IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
3 65-74 LEYDPRLVYD IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
4 89-98 GERTRGRQPG IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
5 97-106 PGDYDDDRRQ IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
6 107-116 PRREEGGRWG IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
7 123-132 REREEDWRQP IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
8 134-143 EDWRRPSHQQ IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
9 143-152 QPRKIRPEGR IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
10 294-303 TPGQFEDFFP IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
11 311-320 SYLQEFSRNT IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
12 325-334 FNAEFNEIRR IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
13 344-353 EQEERGQRRW IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
14 393-402 DITNPINLRE IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
15 409-418 NNFGKLFEVK IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
16 498-507 RRYTARLKEG IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
17 525-534 ELHLLGFGIN IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
18 539-548 HRIFLAGDKD IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
19 551-560 IDQIEKQAKD IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
20 559-568 KDLAFPGSGE IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
21 578-587 KESHFVSARP IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
35_relaxed_rank_1_model_3.pdb



Epitope Reference ( ID 1): D. S. Shin, C. M. Compadre, S. J. Maleki, R. A. Kopper, H. Sampson, S. K. Huang, A. W. Burks, and G. A. Bannon, Biochemical and structural analysis of the IgE binding sites on Ara h 1, an abundant and highly allergenic peanut protein, J. Biol. Chem. 1998, 273, 13753-13759.
SDAP Home Page | Search SDAP | SDAP Manual | SDAP FAQ | Contact  
UTMB | Search | Directories | UTMB Map | News | Employment | Sitemap 
This site published by Surendra Negi
Copyright   2001-2022  The University of Texas Medical Branch. Please review our privacy policy and Internet guidelines.