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Department of Biochemistry and Molecular Biology Sealy Center for Structural Biology Computational Biology


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                SDAP 2.0 - Structural Database of Allergenic Proteins
Go to: SDAP All allergens       Go to: SDAP Food allergens
Send a comment to Werner Braun      Submit new allergen information to SDAP
  
Alphabetical listing of allergens: A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Access to SDAP is available free of charge for Academic and non-profit use.< Licenses for commercial use can be obtained by contacting W. Braun (webraun@utmb.edu). Secure access to SDAP is available from https://fermi.utmb.edu/SDAP

Allergen Info Description
Allergen ID 67
Allergen Scientific Name Cry j 2
Allergen Species - Systematic Name Cryptomeria japonica
Allergen Species - Common Name Japanese cedar, sugi
Allergen Common source tree
Allergen Common name Pinales
Allergen Keywords Polygalacturonase; PG; Pectinase; EC 3.2.1.15
Allergen IUIS (Yes=1, No=0) 1

Cry j 2 : There are iso allergen in the table! Click on each iso-allergen for more information.

ID Allergen Name
1 Cry j 2.0101
2 Cry j 2.0102
Cry j 2 - References
ID Reference
Pubmed ID: 2382797
Sakaguchi M, Inouye S, Taniai M, Ando S, Usui M, Matuhasi T, Identification of the second major allergen of Japanese cedar pollen. Allergy. 1990 May;45(4):309-12.
Pubmed ID: 7926035
Namba M, Kurose M, Torigoe K, Hino K, Taniguchi Y, Fukuda S, Usui M, Kurimoto M. Molecular cloning of the second major allergen, Cry j II, from Japanese cedar pollen. FEBS Lett. 1994 Oct 17;353(2):124-8.
Pubmed ID: 8002972
Komiyama N, Sone T, Shimizu K, Morikubo K, Kino K. cDNA cloning and expression of Cry j II the second major allergen of Japanese cedar pollen. Biochem Biophys Res Commun. 1994 Jun 15;201(2):1021-8.
Pubmed ID: 8564723
Hashimoto M, Nigi H, Sakaguchi M, Inouye S, Imaoka K, Miyazawa H, Taniguchi Y, Kurimoto M, Yasueda H, Ogawa T. Sensitivity to two major allergens (Cry j I and Cry j II) in patients with Japanese cedar (Cryptomeria japonica) pollinosis. Clin Exp Allergy. 1995 Sep;25(9):848-52.
Pubmed ID: 12580914
Tamura Y, Kawaguchi J, Serizawa N, Hirahara K, Shiraishi A, Nigi H, Taniguchi Y, Toda M, Inouye S, Takemori T, Sakaguchi M. Analysis of sequential immunoglobulin E-binding epitope of Japanese cedar pollen allergen (Cry j 2) in humans, monkeys and mice. Clin Exp Allergy. 2003 Feb;33(2):211-7.
Cry j 2 - Protein Sequences
Source Link to source
SwissProt P43212
GenBank 506858
Cry j 2 - Theoretical Model Structure
Model ID PDB file PDB Snapshot PDB Snapshot 90 PDB Snapshot 180 PDB Snapshot 270 3D PDB View
98 GetModel Model Pic Model Pic Model Pic Model Pic Click here
308 GetModel Model Pic Model Pic Model Pic Model Pic Click here
Cry j 2 - Sequence Search :
Sequence Header Sequence
Cry j 2
SDAP Sequence ID: 308
SDAP Allergen ID: 67
MAMKLIAPMAFLAMQLIIMAAAEDQSAQIMLDSVVEKYLRSNRSLRKVEHSRHDAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKNPSAMLLVPGSKKFVVNNLFFNGPCQPHFTFKVDGIIAAYQNPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLSPSAKRKESKSHKHPKTVMVENMRAYDKGNRTRILLGSRPPNCTNKCHGCSPCKAKLVIVHRIMPQEYYPQRWICSCHGKIYHP
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Cry j 2
SDAP Sequence ID: 98
SDAP Allergen ID: 67
MAMKFIAPMAFVAMQLIIMAAAEDQSAQIMLDSDIEQYLRSNRSLRKVEHSRHDAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFTFKVDGIIAAYQNPASWKNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIASCLNDNANGYFSGHVIPACKNLSPSAKRKESKSHKHPKTVMVKNMGAYDKGNRTRILLGSRPPNCTNKCHGCSPCKAKLVIVHRIMPQEYYPQRWMCSRHGKIYHP
View 3D Structure Click here to view 3D model structure of allergen
Fasta Search in SDAP
Blast Swissprot database search at NCBI
Blast PDB database search at NCBI
Blast NR database search at NCBI
Cry j 2 - Statistics from Alphafold ( SI is sequence id, Best template format: PDB_ChainID)
Allergen Name pLDDT Score pTM Score Best PDB template %Seq ID Align length Seq length Query Range Sequence Range E-Value Bit Score Download Model
Cry j 2 85.7 0.71 1BHE_A 32.959 267 514 150 - 404 113 - 363 2.43e-24 105 Download (SI: 98)
Cry j 2 86.35 0.72 1BHE_A 29.781 366 514 71 - 404 19 - 363 1.31e-24 106 Download (SI: 308)
Cry j 2 - Alphafold Theoretical Model Structure
Model Structure
98_relaxed_rank_1_model_4.pdb


Coverage Plddt
Cry j 2 - Protein Sequence Properties!
Protein Sequence Protease cleavage sites PeptideCutter at ExPASy
P43212 Click here to process the data
506858 Click here to process the data
Cry j 2 - PFAM Information
SeqID Aller ID AlnS AlnE EnvS EnvE PFAM ID Family Type Clan Description
98 67 92 414 86 421 PF00295 Glyco_hydro_28 Repeat CL0268 Glycosyl hydrolases family 28
98 67 466 514 465 514 PF17181 EPF Family No_clan Epidermal patterning factor proteins
308 67 92 414 45 421 PF00295 Glyco_hydro_28 Repeat CL0268 Glycosyl hydrolases family 28
308 67 466 514 465 514 PF17181 EPF Family No_clan Epidermal patterning factor proteins
Where: AlnS= Alignment start position, AlnE= Alignment end position, EnvS= Envelope start position, EnvE= Envelope end position.

List of B-cell epitopes predicted by BepiPred method using a window size of 9 amino acids

Cleck here for epitopes predicted by BepiPred method
Cry j 2 - Epitope Information from published data
No. Position Epitope Sequence Epitope Type View epitope Search epitope in SDAP PD search in SDAP Cross-React search
1 96-116 PGNKKFVVNNLFFNGPCQPHF IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
2 166-185 GQCKWVNGREICNDRDRPTA IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
3 324-348 SHIIYENVEMINSENPILINQFYCT IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
4 346-365 YCTSASACQNQRSAVQIQDV IgE Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
1 120-134 VDGIIAAYQNPASWK T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
2 135-149 NNRIWLQFAKLTGFT T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
3 195-209 IIQGLKLMNSPEFHL T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
4 240-254 ASKNFHLQKNTIGTG T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
5 290-304 SRAEVSYVHVNGAKF T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
6 390-404 CKDIKLSDISLKLTS T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
7 400-414 LKLTSGKIASCLNDN T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
8 405-419 GKIASCLNDNANGYF T-cell Click here to find exact epitope Click here to find similar epitopes Click here for Cross-React search
98_relaxed_rank_1_model_4.pdb



Epitope Reference ( ID 33): T. Sone, K. Morikubo, M. Miyahara, N. Komiyama, K. Shimizu, H. Tsunoo, and K. Kino, T cell epitopes in Japanese cedar (Cryptomeria japonica) pollen allergens: choice of major T cell epitopes in Cry j 1 and Cry j 2 toward design of the peptide-based immunotherapeutics for the management of Japanese cedar pollinosis. J. Immunol. 1998, 161, 448-457.
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